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KMID : 0545120160260040717
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Journal of Microbiology and Biotechnology
2016 Volume.26 No. 4 p.717 ~ p.724
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Biological Methanol Production by a Type II Methanotroph Methylocystis bryophila
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Patel Sanjay K. S.
Mardina Primata
Kim Sang-Yong
Lee Jung-Kul
Kim In-Won
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Abstract
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Methane (CH4) is the most abundant component in natural gas. To reduce its harmful environmental effect as a greenhouse gas, CH4 can be utilized as a low-cost feed for the synthesis of methanol by methanotrophs. In this study, several methanotrophs were examined for their ability to produce methanol from CH4; including Methylocella silvestris, Methylocystis bryophila, Methyloferula stellata, and Methylomonas methanica. Among these methanotrophs, M. bryophila exhibited the highest methanol production. The optimum process parameters aided in significant enhancement of methanol production up to 4.63 mM. Maximum methanol production was observed at pH 6.8, 30°C, 175 rpm, 100 mM phosphate buffer, 50 mM MgCl2 as a methanol dehydrogenase inhibitor, 50% CH4 concentration, 24 h of incubation, and 9 mg of dry cell mass ml-1 inoculum load, respectively. Optimization of the process parameters, screening of methanol dehydrogenase inhibitors, and supplementation with formate resulted in significant improvements in methanol production using M. bryophila. This report suggests, for the first time, the potential of using M. bryophila for industrial methanol production from CH4.
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KEYWORD
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Biogas, greenhouse gases, methane, methanol production, Methylocystis bryophila
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